The use of nested Polymerase Chain Reaction (nested PCR) for the early diagnosis of Histoplasma capsulatum infection in serum and whole blood of HIV-positive patients*
Kátia Cristina DantasRoseli Santos de FreitasAdriana Pardini Vicentini MoreiraMarcos SilvaGil BenardCídia VasconcellosPaulo Ricardo Criado
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Abstract:
The aim of the study was to detect the rDNA sequences and their regions in Histoplasma capsulatum, which could be considered species-specific and used as a molecular method for this diagnosis by the technique of nested polymerase chain reaction (nested PCR), employing specific sequences (primers) for H. capsulatum: 18S rDNA region (HC18), 100 kDa (HC100) and the sequence 5.8 S-ITS rDNA (HC5.8). The PCR sequences HC18, HC100 and HC5.8 resulted in a specificity of 100%. The molecular assays may increase the specificity, sensitivity and speed in the diagnosis of Histoplasmosis.Keywords:
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Background.. Histoplasma capsulatum has become an increasingly frequent opportunistic infection among patients whose immune system is impaired either by infection with human immunodeficiency virus (HIV) or by other factors. Because of the existence of this primary pathogen fungus in nature in Turkey has been shown, the possibility of recovering the cases of histoplasmosis and some important notes concerning on its diagnosis have been discussed in the paper. * Anahtar Kelimeler: Histoplasma capsulatum, Histoplazmoz tanimi, Turkiyede Histoplazma capsulatum *Key Words: Histoplasma capsulatum, Diagnosis of histoplasmosis, Turkey
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"Occurrence of Histoplasma Capsulatum Darling, 1906 in Israel, with a Review of the Current Status of Histoplasmosis in the Middle East" published on Jan 1977 by The American Society of Tropical Medicine and Hygiene.
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