Assessment of lifestyle effect on oxidative stress biomarkers in free‐living elderly in rural Japan
Basilua André MuzemboNarongpon DumavibhatNlandu Roger NgatuMasamitsu EitokuRyoji HirotaNarufumi Suganuma
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Background: Oxidative stress is believed to play a crucial role in aging and age‐related diseases, and is widely thought to increase morbidity and mortality in the elderly. Assessment of biomarkers of oxidative stress, such as 8‐isoprostane and 8‐hydroxy‐2‐deoxyguanosine, are considered to be useful in predicting disease risks at the population level. Objective: The aim of the present study was to assess the health status of the elderly by comparing their lifestyles and levels of oxidative stress biomarkers. Methods: We carried out a cross‐sectional study where urine samples from a total of 100 elderly men and women were assayed for 8‐isoprostane, 8‐hydroxy‐2‐deoxyguanosine, selenium, cadmium and creatinine. They were asked to answer a questionnaire that included questions about their lifestyle. Results: Most of the participants were prehypertensive, non‐alcohol users and on a rich plant‐based diet. There were no differences in any biomarkers of oxidative stress between men and women. 8‐Isoprostane was found to correlate positively with systolic blood pressure in women, but not in men. There was a slight increase of 8‐isoprostane in participants with a poor intake of vegetables, and a decrease of 8‐hydroxy‐2‐deoxyguanosine in participants who consumed fish. Multiple regression analysis showed that oxidative stress biomarkers were positively associated with cadmium, and negatively associated with selenium and fish intake in all participants, 89% of which were non‐smokers. Conclusion: Results from the present study show that fish intake has the potential of decreasing oxidative stress among elderly persons. Geriatr Gerontol Int 2012; 12: 547–554.Keywords:
Isoprostanes
Deoxyguanosine
8-Hydroxy-2'-deoxyguanosine
Cross-sectional study
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Biochemical, biophysical and biological studies of oligonucleotides containing lesions at defined sites provide a molecular basis for the effects of DNA lesions. dG (deoxyguanosine) is the most easily oxidized of the four native nucleotides. The chemical reactivity of dG correlates with compilations of mutations, which reveal that a significant fraction of transitions or transversions involve dG. OxodG (7,8-dihydro-8-hydroxy-2´-deoxyguanosine) is widely recognized as an important lesion derived from the oxidation of dG, and significant effort has been expended in studies of its effects on DNA structure and function. Recently, the properties of other lesions derived from dG and/or the oxidation of OxodG have been uncovered. Studies on these lesions reveal that they too are biologically significant.
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【目的】酸化ストレスマーカーである尿中8-ヒドロキシ-2'-デオキシグアノシン(8-OHdG)値について運動に伴う変動を中心に検討した.【対象と方法】健常な学生の協力によりエルゴメーター運動負荷を行い, 安静時, 運動負荷後1時間以内および回復時に, 採尿を行った.尿は検査時まで-80℃に保存し, ELISA法で8-OHdG値を測定した.結果はクレアチニン補正値および体重・時間あたり生成量で評価した.【結果】尿中8-OHdG値に性差はなく, 日内変動の検討ではクレアチニン補正値(μg/g Cr)がほぼ一定で安定していた.尿中8-OHdG値(μg/g Cr)は運動後に有意に上昇し(p<0.01), 回復時には運動前の値に戻った.【結語】尿中8-OHdG値は採尿の簡便さからクレアチニン補正値で表現する方が便利であり, かつ日内変動も小さく, 安定した成績が得られた.運動負荷後1時間以内に尿中8-OHdG値(μg/g Cr)が有意に上昇したことから, 8-OHdGは比較的早く出現することがわかった.
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Oxidative damage from reactive oxygen species including free radicals has been considered to play a vital role in many chronic diseases.8-hydroxy-2′deoxyguanosine(8-OHDG) is one of modified products resulting from DNA oxidative damage,which can be detected by methods with high sensitivity and high selectivity,and is a common used biomarker in detecting DNA oxidative damage.This paper introduces the source,detecting method and apllication of 8-OHDG.
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DNA is subject to constant oxidative damage from endogenous oxidants. The oxidized DNA is continuously repaired and the oxidized bases are excreted in the urine. A simple routine analytical procedure is described for urinary 8-hydroxy-2'-deoxyguanosine, an oxidative DNA damage adduct, as an indicator of oxidative damage in humans and rodents. This adduct was purified from human urine and characterized. The described assay employs a series of solid-phase extraction steps that separate 8-hydroxy-2'-deoxyguanosine from other urinary constituents, followed by analysis by gradient reversed-phase HPLC coupled to a dual-electrode high-efficiency electrochemical detection system. Analysis of urine from three species by this method indicates that mice excrete approximately 3.3-fold more 8-hydroxy-2'-deoxyguanosine than humans (582 vs. 178 residues per cell per day), a result that supports the proposal that oxidative damage to DNA increases in proportion to species-specific basal metabolic rates.
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