Cytotoxic Activity of Tegari ( Dianella nemorosa Lam; Liliaceae) Leaves Methanol Extract from Papua Against Human Cells Lines In Vitro
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Abstract:
Cancer is one of deathly diseases in the world. Many people conversely attempt to employ traditional medicine for primary health care, especially from Plants. Dianella nemorosa Lam. has their own local name in Papua, pra kepey and belongs to the Liliaceae family. It is one of the anticancer plants that is empirical efficacious usage. The aim of the research was to find out cytotoxic activity of methanol extract of D. nemorosa leaves against selected human cell line namely hormone-dependent breast carcinoma cell line (MCF-7), human breast carcinoma cell line (T-47D), colon carcinoma cell line (WiDR) and human-slymphoma cell line (Raji Cell). Leaves powder were extracted using methanol. Cytotoxic assay of the methanol extract was done using MTT [3-(4,5-dimetilthiazol-2-il)-2,5difeniltetrazolium bromida) assay method in vitro . The result indicated that methanol extract of D. nemorosa leaves possessed cytotoxic activity in all tested cancer cell line, with the IC 50 values of 1,794 µg/ml, 1,732 µg/ml, 1,719 µg/ml, and 1,049 µg/ml for MCF-7, T47D, WiDR and Raji cell line, respectively after incubation 24h. This species could be considered as potential sources of anticancer compounds. Further studies are necessary for chemical characterization of the active principles and more extensive biological evaluations.Keywords:
Raji cell
Liliaceae
MTT assay
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Achillea L. (Asteraceae) is a traditional medicinal herb which contains different phenol and flavonoid compounds that are responsible for Achillea pharmacological effects. Turkey is one of the most important centers of diversity for the genus Achillea in the world. The aim of this research was the investigation of the cytotoxic activity of A. coarctata Poir. extract on a human breast cancer cell line (MCF-7). The cytotoxic activity of the extract on the MCF-7 cell line and mouse embryo fibroblast cells (NIH/3T3) were evaluated by 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphynyl tetrazolium bromide (MTT) assay. The extract has cytotoxic activity on MCF-7 cell line with IC50 values 37.39 and 33.98 µg/mL after 24 and 48 h treatments, respectively. The results showed that the extract inhibited significantly MCF-7 cell. Further investigation is required to assess the cytotoxic potential of the extract in cancer therapy, and to isolation and purification of bioactive compounds.
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In this study, we evaluated anticancer activity of methanol extract and purified compound from Loranthus Longiflorus against Human lung cancer cells (A-549), human cervical carcinoma (HeLa) and human hepatocellular carcinoma cell line (HepG2). Cytotoxicity of Loranthus Longiflorus was investigated using MTT assay. Doxorubicin was used as the positive control. MTT assay showed activity against all three tested cell lines. The cytotoxicity activities expressed as percentage of cell viability and the compound was effective on HepG2, HeLa and A-549 cell lines. Cytotoxicity of methanolic extract of LLF against A-549 cell line was measured and the IC50 value of methanol extract of LLF was 17 ± 1.5 µg/ml. In HeLa cell line, the IC50 value of methanol extract of LLF was16 ± 1.0 µg/ml. Cytotoxicity of methanolic extract of LLF against HepG2 cell line was also evaluated. IC50 value of Doxorubicin (standard) was 11 ± 1.0 µg/ml and IC50 value of methanol extract of LLF was 18 ± 0.5 µg/ml. Similarly cytotoxicity showed a significant activity of the compound LLF on HepG2, HeLa and A-549 cell lines. The IC50 values of compound were 13.5 ± 1.5 µg/ml, 15 ± 1.5 µg/ml, 17 ± 0.5 µg/ml against A-549, HeLa and HepG2 cell lines. The present findings confirmed cytotoxic effect of methanol extract and purified compound from Loranthus Longiflorus against A-549, HeLa and HepG2 cell lines.
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Luvunga scandens belongs to the family of Rutaceae which usually inhabit tropical and moist environment. This plant is known as ‘Mengkurat Jakun’ among locals and used traditionally to treat fever and fatigue via decoction. The aim of this study was to investigate the cytotoxic activity of the leaves and stems extracts of L. scandens extract. Extracts of the leaves and stems were obtained from sequential extraction procedures by various organic solvents. All extracts were subjected to cytotoxic study by 3-(4, 5-dimethylthaizol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. In in vitro cytotoxicity assay, all L. scandens extracts exhibited cytotoxicity against human breast adenocarcinoma (MCF-7) and human lung adenocarcinoma (A549) cell lines. The IC50 values of dichloromethane and methanol extracts from the leaves of L. scandens against MCF-7 cell line were 62.5 µg/mL and 88.0 µg/mL, respectively, whereas IC50 of methanol extract from stem was 81.0 µg/mL. All extracts were less active against A549 cell line where IC50 value were not be determined. The present findings revealed the potential of L. scandens as a cytotoxic agent against MCF-7 cell line. However, further studies should be planned to evaluate role of the plant in cytotoxic activity.
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Research is focusing on the search for new types of natural chemotherapeutic agents derived from plants which are proving to be excellent sources of new compounds. The present research article was aimed to study the cytotoxic activity of methanolic extracts of Artocarpus heterophyllus plant by various in vitro cytotoxic assays like MTT and SRB against different cell lines like HEK293, A549, HeLa and MCF-7. The IC50 values of methanolic extract of Artocarpus heterophyllus were found 35.26 µgm/ml and 35.27 µgm/ml against A549 cell line by MTT and SRB assay methods respectively whereas this extract was found to be non toxic to normal cells (HEK293), proved that the methanolic extract exhibited significant anti cancer potential with no toxicity on normal cell line. The methanolic extract had no activity against Hela and MCF-7 cell line.
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The aim of this study was to investigate in vitro cytotoxic activity of four methanolic crude plant extracts against panel cell lines.
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Raji cell
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The genus Scutellaria (Labiatae) is represented by 25 species in Turkish flora. Some of Scutellaria species are used as tonic, wound healing, hemostatic, antioxidant and antitumor in the world. This is a comparative study to evaluate cytotoxic efficacy of three endemic Scutellaria species; S. salviifolia Bentham (SS), S. glaphyrostachys Rech.f. (SG), S. rubicunda Hornem. subsp. brevibracteata J.R.Edm. (SB) against three different cell lines (HEp-2: human cervix epithelial carcinoma, HeLa: human cervix epithelial cancer cell lines and L929: mouse fibroblast non-cancerous cell line) by MTT method. For the activity tests, methanol extracts of the aerial parts were used. IC50 values were found in a range of 378.0–494.7, 381.7–423.7 μg/ mL against HeLa and HEp-2 cell lines, respectively. All extracts showed lower cytotoxicity on L929 cell line than cancer cells with IC50 value (753.0–1524.6 μg/mL). Due to the results, SB was the most effective extract to both cancer cell lines. SS and SG were more sensitive on HEp-2 than HeLa cell line. Our findings indicated that Scutellaria extracts have selective cytotoxic activity on both tested cancer and non-cancerous cell lines. This selectivity is important for discovery of new anticancer agents.
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Tecomastans (L.) (Family:Bignoniaceae) is called as yellow elder in english.Traditionally flowers and bark are used for treatment of various cancers. In current study Crude ethanolic leaf extract of Tecomastans(L.)leaves were examined for their anticancer activity. To determine invitro anticancer activity, different concentrations of crude extract were tested on MCF- 7 cancer cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Tecomastansleaf extract showed a significant antiproliferative activity and a dose dependent effect was observed. Minimum inhibition of 14.6 % was shown by extract at concentration 7.8 µg/ml and maximum inhibition (95.9%) was observed at 1000 µg/ml. The plant extract showed activity in potential range for further investigation on cancer cells.
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Bignoniaceae
Cancer cell lines
MTT assay
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Background: Erythrina is a medicinal plant which is frequently used to treat cancer in Africa. People in Java, however, use Erythrina fusca (cangkring) to treat varicella and measles. Previous works demonstrated that the methanol extract of this plant's leaves induced DNA topoisomerase II mediated DNA cleavage. This activity has been used widely as a target to find anticancer medicine. In order to be scientifically proofed the activity, therefore, it is necessary to analyze directly on the cancer cell-lines. Objectives: To identify the cytotoxicity effect of methanol extract of E. fusca leaves against cancer cell-lines. Methods: Cytotoxicity analysis of methanol extract isolated from E. fusca leaves was carried out against myeloma and HeLa S-3 cancer cell-lines, and to normal mononuclear cell. The level of cytotoxicity was determined by calculating the level of IC50 which was based on the percentage of the cell death following the 24 hours incubation with the extract. Results: It was demonstrated that this methanol extract was cytotoxic to myeloma and HeLa S-3 cell-lines with the IC50 of 0.005 mg/ml and IC50 of 0.08 mg/ml respectively. The percentage of the cell death on treated normal mononuclear cell with the extract, however, was very much low 110%). This was similar to that on the DMSO treated cells. Conclusion: The methanol extract isolated from E. fusca leaves was demonstrated had a selective cytotoxicity effect, as indicated by the level of the IC50 which was higher to myeloma compared to HeLa S3 cell-line, and had much less cytotoxic on normal mononuclear cells. Key words : Erythrina fusca, cytotoxicity, cancer cell-lines, mononuclear cell
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Background and objectives: Cancer is known to be the second cause of death around the world. The most prevalent cancer among women is breast cancer. Use of plant-derived products in cancer treatment may reduce adverse side effects. Extensive research around the world has led to the discovery of herbal compounds that can be used to treat some types of cancer. According to previous studies, the methanol extract of Eupatorium cannabinum has shown cytotoxic effects in some cancer cell lines. In the present study, bioassay guided fractionation and isolation was conducted on E. cannabinum to evaluate the cytotoxic activityin MCF-7 breast cancer cell line. Methods: The Extraction from theaerial parts was performed by maceration method. Isolation and purification of the extracts were performed by column chromatography. The cytotoxic activity of different extracts of E. cannabinum was evaluated against MCF-7 cell line by MTT assay and a compound was isolated according to bioassay guided fractionation. The cytotoxic activity and apoptotic property of the isolated compound was determined. Results: The chloroform extract was the most active one with IC50 of 21.39±3.24 μg/mL followed by the n-hexane and methanol extracts with IC50values of 60.23±2.16 μg/mL and 81.74±3.41 μg/mL, respectively. IC50 of subfractions (1-6) from the chloroform extract were 60.83±2.56 μg/mL, 58.93±2.73 μg/mL, 37.5±3.65 μg/mL, 7.86±1.34 μg/mL, 10.61±2.34 μg/mL and 13.77±4.17 μg/mL, respectively. Eucannabinolide, a sesquiterpene lactone, was isolated from the chloroform extract according to bioassay guided fractionation. Its IC50 was found to be 13±2.45 μg/mL. Eucannabinolide induced 46.91% apoptosis at concentration of 13 μg/mL in MCF-7 cell line in Annexin V/PI assay. Conclusion: Eucannabinolideis a promising candidate for further breast cancer drug discovery studies.
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Cancer cell lines
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Dianella nemorosa Lam. (Liliaceae) is a medicinal plant traditionally used in Papua for treatment of cancer, injury, fracture, inflammation and also for antiseptic. The aim of this study was to evaluate antipriliferative activities of methanol extract of D. nemorosa leaves against HCT+116 (colorectal cell line), C2C12 (adherent mouse myoblast cell line) and 293A (Primary embryonal human kidney transformed with human adenovirus type 5 DNA). Powder of leaves was extracted using methanol. Antiproliverative activities were determined by cell proliferation reagents WST-1 assay for 1 h, 2h and 4h after 72h incubation. The result showed that methanol extract of D.nemorosa leaves showed remarkable antiproliferatiove activities against HTC-116 cell line with IC50 values of 199.31 I¼g/ml (1h), 197.87Â I¼g/ml (2h) and 161.12Â I¼g/ml (4h). The activities against C2C12 cell line resulted in the IC50 values of 405.51 I¼g/ml (1h), 435.12Â I¼g/ml (2h) and 394.38Â I¼g/ml (4h), while the IC50 values for 293A cell line were 580.81Â I¼g/ml (1h), 442.21Â I¼g/ml (2h) and 366.74Â I¼g/ml (4h), respectively. Those results indicated that methanol extract of D.nemorosa leaves posses potential antiproliferative activities against HCT-116, C2C12 and 293A cell line. Further study is necessary to investigate the inhibitory mechanism of methanol extract of D. nemorosa leaves on HCT-116, C2C12 and 293A cell lines. Keywords : Dianella nemorosa Lam, cancer cell, HCT-116, C2C12, 293A
C2C12
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Cancer cell lines
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