Effect of Nicotine on the Differentiation of Human Keratinocytes In Vitro
C. TheiligAugust BerndF. E. GörmarA. Ramírez-BoscáJürgen Bereiter‐HahnBrigitte Keller‐StanislawskiN. RietbrockH. Holzmann
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HaCaT
HaCaT
Human skin
Keratin 8
Keratin 5
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Objective To study the protective effect of astaxanthin on HaCaT keratinocytes injured by UVA induced oxidative stress.Methods Human keratinocytes HaCat were used as an experimental model in vitro.Cell viability was determined by MTT and the amounts of SOD、GSH-px、MDA were measured by biochemical methods.Results Astaxanthin increased the cell viability、SOD and GSHpx,and decreased MDA level of HaCaT keratinocytes.Conclusion Astaxanthin has protective effect on HaCaT keratinocytes injured by UVA induced oxidative stress.
HaCaT
Viability assay
MTT assay
Malondialdehyde
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Objectives: This study was performed to assess the protective effect of Saengmaek-san (SM) on UVB-induced HaCaT cell damage. Methods: The protective effects of Saengmaek-san(SM) were determined by UVB-induced HaCaT assay. We assessed protective effects of Saengmaek-san (SM) on LDH release and nitrite release from HaCaT. And COX-2, Bcl-2, Bax, , c-jun, c-fos, NF-kB, iNOS, Bcl-xL gene expression were determined in HaCaT using real-time PCR method. Results: 1. SM inhibited LDH-release, nitrite production in UVB-exposed HaCaT. 2. SM suppressed the gene expression of COX-2, in UVB-exposed HaCaT. 3. SM increased the gene expression of Bcl-2, Bax, Bcl-xL family protein in UVB-exposed HaCaT. 4. SM suppressed the gene expression of c-jun, c-fos, NF-kB in UVB-exposed HaCaT. Conclusions: The study showed SM inhibited the cell damage in UVB-exposed HaCaT.
HaCaT
Cell damage
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Objective To evaluate the effects of total glucosides of paeony(TGP) on cell proliferation of human HaCaT keratinocytes and the expression of ICAM-1, and explore the signaling pathways which may be involved in the process. Methods HaCaT cells were stimulated by 500 U/mL IFN-γ, then incubated with TGP of various concentrations(0.5 to 312.5 μg/ml). MTT assay was performed to detect the cell proliferation of HaCaT cells. The expression of ICAM-1 in HaCaT cells was evaluated by enzyme linked immunosorbent assay(ELISA) and fluorescent immunohistochemical technology. Results The proliferation of HaCaT cells was promoted by TGP of low concentrations(0.5 to 25.0 μg/ml), but inhibited by TGP of high concentration(62.5 to 125.0 μg/ml). TGP of low concentrations(0.5 to 25.0 μg/mL) significantly reduced the expression of ICAM-1. Conclusion TGP significantly inhibited the upregulated ICAM-1 secretion of HaCaT cells which induced by IFN-γ, that may be one of the anti-inflammatory mechanism of TGP.
HaCaT
MTT assay
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HaCaT
Opiate
A431 cells
Receptor expression
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We have studied the gap junctional intercellular communication (GJIC) of immortalized and tumourigenic human keratinocyte cell lines and of a spontaneously immortalized non-tumourigenic and a highly differentiating keratinocyte cell line (HaCaT) as the control. In homologous cultures, the GJIC capacity of five squamous cell carcinoma-derived cell lines was 1-27% that of the HaCaT cells. Ha-ras-transfected HaCaT cells with tumourigenic potential and an SV40 DNA-immortalized cell line had markedly reduced GJIC capacities. Northern analysis and immunohistochemistry showed that connexin (Cx) 43 is the major gap junction protein expressed in the communicating cells. They do not express Cx 26 or 32. The low or absent communication observed in certain cell lines was due in some to a lack of Cx 43 gene expression, but in others to aberrant localization of the gap junction protein. GJIC of these cell lines, as well as that of primary normal human epidermal keratinocytes, was susceptible to 12-O-tetradecanoylphorbol-13-acetate-mediated inhibition. Moreover, GJIC of HaCaT cells and their tumourigenic derivatives is Ca(2+)-dependent. These results, when compared with those previously obtained for mouse keratinocyte cell lines, reveal that GJIC of human keratinocytes was correlated to the degree of differentiation and is controlled in a similar way to that of murine keratinocytes. Aberrant GJIC seems to be a common feature of human and murine skin carcinogenesis.
HaCaT
Immortalised cell line
Cell type
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Phellinus igniarius is widely used for anti-oxidation in China, and the effect of anti-oxidation of polysaccharides from Phellinus igniarius fruiting body (PIP) was studied in this article. AFM was used to scan the different groups of HaCaT cells. The HaCaT cells height, adhesion and Young's modulus were determined. In this study, the height of HaCaT cells were close gradually to the normal HaCaT cells after ethanol induced oxidative damage when cultured with PIP for 6-24 h, and the results were time-dependent. The adhesion of HaCaT cells cultred with PIP for 18h was closer to the normal cells than that with PIP for 12h. When the cells cultured with the PIP for 12-24 h, the HaCaT cells Young's modulus with ethanol induced oxidative damage was close to that of normal HaCaT cells. These results demonstrated that the effect of PIP against oxidative damage of HaCaT cells.
HaCaT
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HaCaT
Biocompatibility
Viability assay
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HaCaT
Sulfur mustard
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ABSTRACT Objectives ：This study was carried out to investigate anti-oxidative effect s of Taraxaci Herba (TH) and protective effects on Human HaCaT keratinocyte.Methods : Anti-oxidative effects were measured by estimating the amount o f total phenolics and flavonoids. In addition, DPPH free radical scavenging activitie s were estimated. Protective effects of TH on HaCaT keratinocytes against oxidative stress induced by h ydrogen peroxide were also measured. Results : In our results, treatment with TH did not show cytotoxicity on HaCaT keratinocyte beneath the concentration of 200 ㎍/㎖. 42.64±1.90 ㎍/㎖ of total phenolics and 28.09±1.84 ㎍/㎖ of flavonoids was detected from TH ethanol extract. In addition, D PPH free radical scavenging activities of TH were elevated in dose-dependent manner. In add ition, The value of half maximal inhibitory concentration (IC 50 ) was 165.5 ㎍/㎖. Finally, TH showed protective effect against cell death of HaCaT cell induced by hydrogen peroxide significantly.Conclusions : In conclusion, these results suggest that TH may have anti-oxidantic action in human skin and also suggest the possibility as cosmetic material.Key words : Taraxaci Herba; anti-oxidative effect; HaCaT keratinocyte
HaCaT
Malondialdehyde
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