Overview of Callus (Tissue) and Organ Culture
1
Citation
2
Reference
10
Related Paper
Keywords:
Callus
Organ culture
This experiment was knew effect of: a) Plant Growth Regulator (ZPT) 2,4-Dichlorofenoxyacetic acid, b) Different explant, c) interaction of ZPT 2,4-D and different explant on the callus induction of potato plant varietas Granola. This experiment was performed on November 2013 – March 2014 in laboratory Tissue Culture YAHDI zone of Perum Pelabuhan in Jl. Lambung No. 16 Medan Marelan. Design of experiment is Completelly Randomized Design (RAL) factorial. First factor is ZPT 2,4-D with 4 stage treatment (0, 1, 2, 3 ppm) and second factor is different explant include root, stem and leaf. Total Combination of treatment is 12 with 4 repetition. Observation did 28 day. Parameter observed were time of calus formed, callus teksture, callus colour, callus biomass, callus wide surface. Result of experiment: there is effect very significant a) ZPT 2,4-D, b) different explant, c) interaction of ZPT 2,4-D and different explant on the callus of biomass and wide surface. Explant of stem potensial to form callus on 1 and 2 ppm 2,4-D treatment on 14 day induction. Explant of root potensial to formed callus 3 ppm 2,4-D treatment on 15 day induction and 2 ppm 2,4-D treatment on 16 day induction. Explant of leaf isn’t form callus. Callus colour from explant of root with 2 and 3 ppm treatment is result white-chocelate colour. Explant of stem with 1 ppm 2,4-D is result white-yellow colour, 2 and 3 ppm 2,4-D treatment is result white-green colour. All of callus having friable tekstur. Treatment of 3 ppm ZPT 2,4-D and explant of stem result higher of callus biomass is1,14 gram with wide surface is 1,60 cm2.
Callus
Cite
Citations (0)
The aims of this research were to evaluate the effect of explant types and several kinetin concentrations on in vitro induction and growth of callus vetiver (Vetiveria zizanioides (L.) Nash). Crown and tiller of vetiver were cultured on Murashige and Skoog's (MS) media supplemented with combination of 2,4-D 0.75 ppm and several kinetin concentrations (0, 0.3, 0.5, 0.75, and 1) ppm. The induction and growth of callus were influenced by type of explant and concentration of kinetin. Formation and growth of callus on tiller explant were faster than crown explant. Callus on tiller explant were formed one week after culture, while callus from crown explant were formed at four weeks after culture. Callus growth on tiller explant also was better than crown explant. Eight weeks after culture, callus fresh weight from tiller explant was 0.35 ± 0.09 g, while callus fresh weight from crown explant was only 0.16 ± 0.08 g. The addition of kinetin in the medium combined with 2,4-D was able to increase callus growth and the optimum concentration of kinetin used was 0.5 ppm. The addition of kinetin more than 0.5 ppm in the medium decreased the callus fresh weight.Keywords: callus, crown, in vitro culture, tiller, Vetiveria zizanioides.
Kinetin
Callus
Cite
Citations (1)
Organ culture
Cite
Citations (2)
Callus
Cite
Citations (6)
Organ culture
Cite
Citations (9)
[Objective]The study aimed to provide the scientific direction of rapid propagation technique.[Method] Taking leaf and hypocotyl of Limonium bicolor as the explant,the system of rapid propagation were established,the influences of hormone concentration to induce the tissue culture and adventitious buds were studied.[Result] Different concentration culture medium can induce the explants grew out tissue culture and adventitious buds,A,B,H,I groups of rooting medium can induce adventitious buds generated adventitious roots,the other groups had no adventitious roots.[Conclusion] Taking cotyledons as explant,the best induction medium of tissue culture and adventitious buds were MS0+0.7 mg/L 6-BA+0.1 mg/L NAA,MS0+0.5 mg/L 6-BA+0.1 mg/L NAA respectively;with hypocotyl as explant,the best induction medium of tissue culture and adventitious buds was MS0+0.3 mg/L 6-BA+0.5 mg/L NAA.The best medium for inducing adventitious roots was MS0.
Cite
Citations (0)
Abstract The purpose of this study was to determine the effect of explant types and plant growth regulators on callus induction of geranium ( Pelargonium graveolens L’Her). Callus induction was carried out by culturing leaf and petiole explants from ex vitro geraniums on MS + 0.5 mg/l 2.4-D + 0.1 and 0.3 mg/l Benzyladenine (BA) or Kinetin. Each culture was carried out in 5 replications, cultures were incubated in rooms 25°C and 600 lux. The parameters days for callus induction and percentage of callus formation were observed. Geranium callus formation was influenced by types of explants and plant growth regulators. Petiole explants was able to induce callus better than leaf explants. Days for callus induction at second week and percentage of callus formation of petiole explants were 58 and 58% compared to leaf explants were only 53 and 54%. Plant Growth Regulators BA combined with 2.4-D was able to induce callus better than kinetin. The best callus formation was produced from petiole explants cultured on MS + 0.5 mg/l 2.4-D + 0.3 mg/l BA by 84%.
Callus
Kinetin
Petiole (insect anatomy)
Pelargonium
Cite
Citations (1)
The present research work aimed to shedding light on the effect of various plant growth regulator namely 2,4 dichlorophenoxy acetic acid (2,4-D) and �-Naphthalene Acetic Acid (NAA) on callus induction of ricinus commun. Callus culture were initiated from cotyledonary leaf and root segments explants from in vitro Ricinus communis L seedling . After four day of inoculation callusing was observed from cotyledonary leaf segment explants, after about one week from root segment explant. Auxins 2,4-D and NAA effecting in callus agitationbut 2.5 mg/L 2,4-D proved to be more effected for induction of callus in R. communis L. Individual treatment of NAA reveal low effectiveness for callus induction. Among all treatment media comprising 2,4 D (2.5 mg/l) showed superior response of callus degree (4 .0±0.0) with cotyledonary leaf segment explant and at this concentration, maximum amount of callus was observed whereas, root segment explants showed (2.7±0.19 ) degree of callus response in the same concentration . The callus from cotyledonary leaf segment explants was Light brown colour with compact nature in media fortified with 2,4 D while in media fortified with NAA callus was Light green colour with Friable nature. The callus from root segment explants was creamy to yellow colour with compact nature with the both tested auxin.
Callus
Cite
Citations (3)
Organ culture
Cite
Citations (15)
Ajowan (Trachyspermum ammi) has been considered as an important medicinal plant because it contains many alkaloids such as Thymol. In vitro culture of Ajowan provides new tissue sources such as callus, cell suspension and seedlings to produce secondary metabolites. The present study describes callus production optimization procedures experiment that was a factorial experiment based on completely randomized design at three levels with four explants (root, shoot, leaf and cotyledon) on Murashige and Skoog (MS) medium supplemented with different concentrations of BAP (0.25, 0.5 and 1 mg.L-1) and 2,4-D (2, 4 and 8 mg.L-1). Comparison of means showed that the maximum callus production was obtained from shoot explants, the cotyledon and leaf explants were in the second orders. In overall, 0.25 mg.L-1 BAP with 2 mg.L-1 2,4-D concentrations proved to be optimal for the production of maximum callus and also were more effect on callus weight, callus volume and callus color. The best explant based on callus weight was cotyledon explant and for callus volume was shoot explant. The result were shown that the effective hormone combination and explant was 2 mg.L-1 2,4-D with 0.25 mg.L-1 BAP concentrations were more effect on callus induction and shoot explant, respectively.
Callus
Cotyledon
Cite
Citations (21)