Acute toxicity and safety evaluation of four organosilicon surfactants to zebrafish.
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Abstract:
In order to assess the effect of organosilicon surfactant as a novel adjuvant for agrochemical to aquatic organisms,the acute toxicity of four organosilicon surfactants SUSHENYIHAO,High-Speed,JIEXIAOLI and JIERUN were studied by semi-static method using zebrafish(Brachydanio rerio) as test organism.Safety evaluation has also been conducted.The results showed that the LC50 value of SUSHENYIHAO,High-Speed,JIEXIAOLI and JIERUN to zebrafish in 96 h were 135.0,17.4,11.3 and 5.9 a.i.mg/L,respectively.The acute toxicity of SUSHENYIHAO,High-Speed and JIEXIAOLI were low toxicity.The acute toxicity of JIERUN was middle toxicity.Keywords:
Organosilicon
Static testing
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A novel rat hepatocidal test, based on morphological changes in monolayer culture and the percentage of lactate dehydrogenase (LDH) released into the medium after exposure to culture filtrates of Listeria spp. was used to determine listerial toxicity and pathogenicity. Primary cultures of rat hepatocytes exposed to brain heart infusion (BHI) culture filtrates from ATCC strains of Listeria monocytogenes and L. ivanovii, released 91–92% and 95% of LDH after 3 h and 18.5 h, respectively. Cultured monolayers changed from normal hepatocytes into nonviable round forms. Brain heart infusion broth and BHI culture filtrates of other Listeria spp. were nontoxic to hepatocytes. The rat hepatocidal test is a quantitative and rapid system for studying listerial toxicity and pathogenicity.
Brain heart infusion
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Abnormality
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