Indomethacin solubilization induced shape transition in CnE7 () nonionic micelles

AOT/イソオクタンを用いた逆相ミセル系において, ミセル粒径分布の定量化, タンパク質のミセル内への可溶化モデルについて検討した.さらにこのモデルに基づき, タンパク質混合物の相互分離について実験的に検討した.ミセル粒径分布は, ミセル相AOT濃度によらず, 水相塩濃度により決まる含水率によって相関できた.またタンパク質のミセル内への溶解特性は, ミセル粒径分布に基づき, 界面に吸着したタンパク質が, それより大きいミセルと衝突することによって, 可溶化されると仮定する簡単なモデルにより, 定量化することができた.またミセル-タンパク質間の静電的な相互作用とミセル粒径分布との関係についても検討した.さらに, このモデルの応用として, Lysozyme, α-Chymotrypsin, BSA の相互分離を行った.

10.1252/kakoronbunshu.16.763

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Effect of Pressure on the Solubilization of a Fluorescent Merocyanine Dye by a Nonionic Surfactant

The Journal of Physical Chemistry B (2011)

Mohammed A. H. Alamiry Andrew C. Benniston Anthony Harriman

The target dye, which is a derivative of Merocyanine 540 bearing a naphthoxazole headgroup, persists as a monomer in ethanol solution but dimerizes in water under ambient conditions. Analysis of the absorption spectrum indicates that the dimer has an oblique geometry with the two molecules being held at an angle of ca. 55°. Applying high pressure to the system forces the two molecules into closer contact, resulting in a decreased partial molar volume of 3.1 cm(3). One molecule of the monomeric dye enters a neutral micelle formed from Triton X-100, where it is highly fluorescent and free of exciton coupling. The result of applied pressure on these latter systems depends on the concentration of surfactant. Above the critical micelle concentration (CMC), applied pressure has little effect other than to increase the viscosity inside the micelle. At very low surfactant concentration, applied pressure forces monomeric dye into the dimeric form, as observed in the absence of Triton X-100. It is notable, however, that the pressure effect on the dimerization constant is exaggerated in the presence of surfactant. At intermediate surfactant concentrations, applied pressure leads to a marked change in the CMC. In particular, applied pressure reduces the partial molar volume of the micelle by ca. 7.9 cm(3) and induces micelle formation at relatively low concentration of surfactant. For example, the CMC falls from ca. 250 μM at atmospheric pressure to only 50 μM at 460 MPa.

Merocyanine

Nonionic surfactant

10.1021/jp2096955

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Research advances in enzyme solubilization reaction in reverse micelle

Science and Technology of Food Industry (2011)

Fusheng Chen

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Fluctuating micelles: a theory of surfactant aggregation part 1. Nonionic surfactants