Antimicrobial systems of the surgical wound
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Wound infection
The bacteria Streptococcus thermophilus belongs to the group of lactic acid bacteria, where the research aims to identify the role of Biosurfactant produced from the bacteria Streptococcus thermophiles for its importance in limiting the growth of Staphylococcus aureus and its effect on some of the physiological parameters of the white rats male ، Streptococcus thermophilus were isolated from local white cheese, and then 120 clinical samples (Wounds, Burns, Blood) were collected. Biosurfactant was extracted from Streptococcus thermophilus and its inhibitory activity was evaluated against Staphylococcus aureus. As well as, its resistance to some antibiotic effects was studied and the effect of Biosurfactant on some physiological parameters in white rats male. ،22 infected samples were obtained, distributed between (11,8, and 3) for each of the (Wound, Burn, and Blood) samples, respectively. The results also showed resistance of bacteria S. aureus to some of the antibiotics used in the study, the results of the statistical analysis to evaluate the inhibitory activity of the Biosurfactant towards S. aureus showed a significant increase at a significant level (P
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Penicillin binding proteins
Protein A
Meticillin
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Staphylococcus aureus is an important pathogen implicated in various diseases, including staphylococcal food poisoning. Bacteriocins are considered safe and effective antimicrobial substances for the prevention of the growth of pathogenic bacteria. In this article, we describe the purification and characterization of pasteuricin, a novel bacteriocin produced by Staphylococcus pasteuri RSP-1. A cell-free supernatant of S. pasteuri RSP-1 exerted strong antimicrobial activity against staphylococci, including methicillin-resistant S. aureus (MRSA), and gram-positive bacteria. The loss of antimicrobial activity upon treatment with proteolytic enzymes confirmed the proteinaceous nature of pasteuricin. A rapid and pronounced bactericidal effect of pasteuricin was confirmed by a live-dead bacterial viability assay. To our knowledge, pasteuricin is the first reported S. pasteuri bacteriocin that inhibits S. aureus. Because pasteuricin is characterized by strong antimicrobial activity and high stability, it has potential as an alternative antimicrobial agent to antibiotics.
Proteolytic enzymes
Pathogenic bacteria
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Objective:To study the antibiotic activities of Qingjie Fanggan Particles in vivo and in vitro.Method:The experiment adopted standard tube double dilution method to measure the Minimal Inhibitory Concentration(MIC)of Qingjie Fanggan Particles and used the flat turn method to measure minimum bactericidal Concentration(MBC),observing its antibacterial effect on staphylococcus aureus,staphylococcus epidermidis and betahemolytic streptococcus in mice.Results:Antibiotic experiment in vitro showed that Qingjie Fanggan Particles had antibiotic effect in varying degrees on common bacterium of upper respiratory tract,especially on staphylococcus aureus,staphylococcus epidermidis and beta hemolytic streptococcus.Meanwhile,it also had inhibitory effect on pseudomonas aeruginosa and candida alicans in some degree.Antibacterial effect in vivo showed that the group infected by staphylococcus aureus which was given 8g/kg and 4g/kg of Qingjie Fanggan Particles had conspicuously reduced the mortality and prolonged survival period.Conclusion:Qingjie Fanggan Particles has some antibiotic activities in vivo and in vitro.
Minimum bactericidal concentration
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Fourteen mupirocin-resistant Staphylococcus aureus strains were isolated over 18 months; 12 exhibited low-level resistance, while two showed high-level resistance. Highly mupirocin-resistant strains contained a large plasmid which transferred mupirocin resistance to other S. aureus strains and to Staphylococcus epidermidis. This plasmid and pAM899-1, a self-transferable gentamicin resistance plasmid, have molecular and biologic similarities.
Mupirocin
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A latex agglutination test system (Rapid Mastitis Test [RMT]; Immucell, Portland, Maine) containing reagents for the identification of Staphylococcus aureus and Streptococcus agalactiae from bovine intramammary infections was evaluated with 527 staphylococcal and 267 streptococcal isolates. The RMT Staphylococcus aureus reagent detected 94.2% of 242 Staphylococcus aureus isolates, 80% of 25 Staphylococcus intermedius isolates, and 42.8% of 21 tube coagulase-positive Staphylococcus hyicus isolates. All Streptococcus agalactiae isolates were correctly identified by the RMT Streptococcus agalactiae reagent. Cross-reactions were observed with one Streptococcus dysgalactiae and three Streptococcus uberis strains. The RMT was found to be an acceptable method for the detection of Staphylococcus aureus and Streptococcus agalactiae isolated from bovine mammary glands. The occurrence of coagulase-positive staphylococci other than Staphylococcus aureus requires biochemical testing for species level identification.
Streptococcus dysgalactiae
Streptococcus uberis
Staphylococcus intermedius
Coagulase
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هدفت الدراسة الى تقييم حدوث التلوث المايكروبي في مجمدات حفظ الاغذية في بعض الاسواق المحلية في مدينة بغداد/ العراق، فضلا عن دراسة تلوث ايدي العاملين في الاسواق وإمكانية تسببها في نقل التلوث للأغذية. أذ تم جمع (30) عينة من الثلج المتواجد في مجمدات حفظ الاغذية من الاسواق المحلية وبشكل عشوائي فضلا عن أخذ (30) مسحة لأيدي العاملين من الاسواق نفسها في آن واحد. و أجري الفحص الميكروبي لعينات الثلج فضلا عن مسحات أيدي العاملين وتم عزل وتشخيص البكتريا من خلال الفحوصات الميكروبيولوجية والكيموحيوية المتبعة. وأظهرت نتائج الفحص الميكروبي عزل اعداد من البكتريا في عينات الثلج الماخوذة من مجمدات حفظ الاغذية, إذ تم عزل اعلى عدد من بكتريا الزوائف الزنجارية Pseudomonas aeruginosa ( 12)عزلة في عينات الثلج تلتها بكتريا Klebsiella spp إذ بلغت (7) عزلات , تلتها المكورات العنقودية الذهبية Staphylococcus aurous ٳذ بلغت (4) عزلات, في حين تساوى عدد عزلات البكتريا التابعة لكل من Staphylococcus epidermidis و Salmonella spp و Streptococcus fecalis و Escherichia coli بلغ (2) عزلة لكل منها، وعزلت (عزلة واحدة) من بكتريا O157:H7) Escherichia (coli . أما في مسحات أيدي العاملين فقد كانت أعداد العزلات لكل من البكتريا Klebsiella spp و Escherichia coli و Staphylococcus aurous و Streptococcus fecalis وStaphylococcus Epidermidi و O157:H7 Escherichia coli هي (10، 10، 7، 5، 4، 2) على التوالي , ولم تواجد بكتريا السالمونيلا وبكتريا الزائفة الزنجارية في مسحات أيدي العاملين.
Klebsiella
Escherichia
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Micrococcus
Chocolate agar
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An extracellular bacteriocin of Staphylococcus epidermidis isolated from dental plaque was purified and characterized. Its molecular mass was 3500 Da and pI was 10.5. This bacteriocin inhibited the growth of Staphylococcus aureus, Streptococcus salivarius , and Streptococcus mitis , but Streptococcus mutans, Streptococcus sanguis and other oral indigenous bacterial species examined were not inhibited. The mode of inhibition was found to be bacteriostatic.
Streptococcus salivarius
Streptococcus mitis
Dental plaque
Streptococcus sanguinis
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