The Ciona Notochord Gene Regulatory Network

Complex gene regulatory networks are at the heart of cell fate specification and differentiation. The simple chordate Ciona has remarkable advantages for the dissection of these regulatory networks, including a stereotypically chordate but extremely small and simple embryo, a streamlined and compact genome, and highly efficient transgenesis by electroporation. Here we use the Ciona notochord as an example of how these characteristics can be exploited to understand both the early network controlling cell fate as well as the tissue-specific network controlling notochord differentiation and morphogenesis.