Effects of Yak skin gelatin on platelet activation

Modern studies shown that gelatin is not only a good hemostatic material, but also a food additive with a broad use. Yak skin gelatin is a new generation of gelatin resources, but studies were not involved on its oral coagulant effects. Bearing in mind the central role of platelets in hemostasis, we aimed, in this study, to examine the pharmacodynamics differences between different molecular Yak skin gelatins on platelet activation. The hemostasis effect of different molecular weight distribution Yak skin gelatins were evaluated for bleeding time (BT), clotting time (CT), and platelet activity by measuring the contents of P-selectin, platelet membrane glycoprotein Ⅰa/Ⅱa (GP Ia/IIa), platelet membrane glycoprotein IIb/IIIa (GP IIb/IIIa) and platelet membrane glycoprotein Ⅳ (GP Ⅳ). Intragastric-administrated of Yak skin gelatin resulted in a significant short of the CT and BT, increase the contents of P-selectin, GP Ia/IIa, GP IIb/IIIa and GP Ⅳ in all groups, in comparison to the control group. The strongest activation of platelet of Yak skin gelatin was observed on the Yak skin gelatin whose molecular weight between 0.1μm and 0.22μm may be in response to its promotive activity on the early stages of platelet activation by improving GP IIb/IIIa and GP Ⅳ levels. While, in measuring the established indicator of platelet activation-platelet activation-dependent granule membrane protein (CD62P), the promotion was observed with all the molecular weight range of Yak skin gelatins. In brief, Yak skin gelatin has hemostasis effect, and Yak skin gelatin fractions between 0.1μm and 0.22μm were the primary reasons to bring about hemostasis by excessively promoting platelet membrane glycoprotein activities and strengthening platelet function.