Application of Suspension Array for Simultaneous Detection of Antibiotic Residues in Raw Milk

Suspension array technology enjoys great potential in simultaneous detection of drug residues in the field of food safety. A monoclonal antibody against gentamycin was produced and a bead-based indirect competitive fluorescent immunoassay was developed using the suspension array system for the simultaneous detection of antibiotic residues such as gentamycin and kanamycin in raw milk. The limit of detection (LOD) for kanamycin and gentamycin were 0.5 ng/mL and 4.1 ng/mL in a phosphate-buffered saline (PBS), 2.2 ng/mL and 12.2 ng/mL in milk. The monoclonal antibodies used for the multiplexed assays showed no significant cross-reactivity with other aminoglycoside antibiotics. To validate the method, milk samples spiked with gentamycin or kanamycin were detected using suspension array technology and conventional enzyme-linked immunosorbent assay (ELISA) in parallel. The good correlation between the obtained results showed that the method is feasible in actual application, and it also laid a firm foundation fo...