Imidazolium-dysprosium-based magnetic NanoGUMBOS for isolation of hemoglobin

Abstract A novel imidazolium-dysprosium-based magnetic nanomaterial, i.e. [C 16 mim] 5 [Dy(SCN) 8 ] nanoGUMBOS ( nano materials fabricated from a g roup of u niform m aterial b ased on o rganic s alts), was prepared using a facile method for selective hemoglobin (Hb) isolation. In this nanomaterial, the imidazolium cation serves as a selective Hb affinity group, while dysprosium contributes paramagnetic properties. Through a combination of the advantages of ionic liquids, magnetic adsorbent, and nanoscale solid phase extraction, [C 16 mim] 5 [Dy(SCN) 8 ] nanoGUMBOS exhibit great selectivity toward Hb and a favorable extraction efficiency of 95.4% when 1 mL of 100 μg/mL Hb solution is processed with 0.6 mg of [C 16 mim] 5 [Dy(SCN) 8 ] nanoGUMBOS. As the Hb concentration increased to 800 μg/mL, the adsorption capacity approached ∼840 μg/mg. The adsorbed protein is recovered with an elution efficiency of 87% by using 1% SDS solution. This novel nanoGUMBOS solid-phase extraction procedure was successfully applied to selective isolation of Hb from human whole blood and verified using SDS-PAGE. This simple strategy is a novel approach towards fabrication and use of a nanoadsorbent for selective isolation of proteins.