3D porous acellular cartilage matrix scaffold with surface mediated sustainable release of TGF-β3 for cartilage engineering

Abstract Acellular tissue matrix scaffolds are much closer to tissue’s complex natural structure and biological characteristics, thus assess great advantages in cartilage engineering. We used rabbit costal cartilage to prepare acellular microfilaments and further 3D porous acellular cartilage scaffold via crosslinking. Poly(L-lysine)/hyaluronic acid (PLL/HA) multilayer film was then built up onto the surface of the resulting porous scaffold. Furthermore, TGF-β3 was loaded into the PLL/HA multilayer film coated scaffold to obtain a 3D porous acellular cartilage scaffold with sustained releasing of TGF-β3 up to 60 days. The success of this project will provide a new way for the treatment of articular cartilage defects. Meanwhile, the anchoring and on-site sustained releasing of growth factors mediated by polyelectrolyte multilayered film can also provide a new method for improving the biocompatibility and the biofunctionality for other implanted biomaterials.