Analysis of Hypertrophic Scar Fibroblasts Expression Fingerprint Using cDNA Microarray

The expression fingerprint of HS fibroblasts was analyzed by cDNA microarray, using mRNA derived from multiple tissues as a contrast. The expression fingerprint of HS fibroblasts was similar to that of normal fibroblast post stimulated by TGF beta3. The expression pattern between the HS and the normal fibroblasts post stimulated was correlated to expression pattern between HS fibroblasts prior and post stimulated by TGF beta3. The expression of 620 genes related to cell cycle, cytokine, oncoprotein and apoptosis was up regulated or down regulated at least 3 times. However, the expression of type Ⅰ and Ⅲ collagen was not changed. The results suggest: (1)The HS fibroblast was in a stress status; (2) Over expression of collagen type Ⅰ and Ⅲ was not necessary for HS formation.