Solubilization and partial purification of squalene synthase from daffodil microsomal membranes.

Abstract A squalene synthase was solubilized from daffodil ( Narcissus pseudonarcissus L. ) microsomes with CHAPS, a zwitterionic non-denaturating detergent. By successive chromatography on DEAE Sephacel and APP Sepharose a fraction enriched in this enzyme (21-fold) was prepared.