Amino acid sequence of the center of the arginine-lysine-rich histone from calf thymus. The total sequence.

Abstract The amino acid sequence of the center of the argininelysine-rich histone (residues 30 to 81) from calf thymus has been determined. This sequence completes the structure of the whole histone by overlapping the 29-residue amino-terminal fragment (Olson, M. O. J., Sugano, N., Yeoman, L. C., Johnson, B. R., Jordan, J., Starbuck, W. C., and Busch, H. (1972) Physiol. Chem. Phys., 4, 10–16) and the 48-residue carboxyl-terminal fragment (Sugano, N., Olson, M. O. J., Yeoman, L. C., Johnson, B. R., Taylor, C. W., Starbuck, W. C., and Busch, H. (1972) J. Biol. Chem., 247, 3589–3591). The complete sequence of the arginine-lysine-rich histone is: [see PDF for equation] Clusters of basic, acidic, and hydrophobic residues were found in this sequence. The basic clusters are underlined. An acidic cluster was observed in the carboxyl-terminal fragment (residues 90 to 93). A very hydrophobic region was present in the sequence from residue 49 to 55. No modified amino acids were found with the exception of the acetylated amino terminus.