[Extraction of fatty constituents from Tabanus bivittatus and their analysis by GC-MS].
2013
OBJECTIVE: To optimize the extracting condition of fatty constituents from Tabanus bivittatus and analyse by GC-MS. METHODS: Taking fatty yield as index, the extraction technology parameters of fatty constituents of Tabanus bivittatus ere optimized by single factor test and orthogonal test. RESULTS: The optimum extraction parameters were as follows: the ratio of acetone to petroleum ether 1: 2, extraction time 2 h, solid-liquid ratio 1: 50. Twenty-one fatty acids were identified by GC-MS. CONCLUSION: The process is reasonable and with good reproducibility. The main components of fatty acids in Tabanus bivittatus are palmitoleic acid, palmitic acid, linoleic acid, oleic acid and stearic acid.
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