Abstract 16120: Extracellar Matrix Preserving Decellulerized Heart Valve Maintain Valvular Function in vivo by Enhancing Functional Cell Recellularization

Background: Maintanance of the valve function and the growth of the implanted heart valves are of great concerns for the treatment of heart valve insufficiency in congenital heart disease. Recently, several reports suggest that the fresh, not for freezing, decellularized heart valves maintained their structure and function after the implantation. However, the detailed process of recellurlarization of scaffold tissue after implantation remain unclear. We examined the long-term function of fresh decellularized heart valves implanted in the pulmonary valve position of porcine hearts in vivo, together with the recellularization and growth potential of the valve scaffolds. Methods: We used the pulmonary heart valve from heart transplant recipient heart or from mini-pigs. The valve were decellularized with 0.5% sodium deoxycholate and 0.5% sodium dodecyl sulfate with shaking condition for 36 hours. We implanted theses valves to mini-pigs in orthotropic position with cardiopulmonary machine(human heart valve: 5(...