Preparative Purification of Pseudomonas aeruginosa Bacteriophages via the Combination of Gel-Permeation and Anion-Exchage Chromatography

We developed and described a method for the purification of Pseudomonas aeruginosa bacteriophages that consists of sequential steps of size-exclusion and anion-exchange chromatography. The technique was tested on five bacteriophages of different size and morphology. Similar results were obtained, which allows the proposed approach to be considered universal and highly scalable.