Association of genetic polymorphisms in selenoprotein GPX1 and TXNRD2 with genetic susceptibility of gastric cancer

Objective this study examined whether the two polymorphisms of GPXl (198Pro→Leu) and TXNRD2 (370Lys→Arg) contributed alone or in combination, to the risk of gastric cancer development. Methods A total of 361 patients with gastric cancer and 363 cancer-free controls were recruited and their genotypes of the two polymorphisms were determined by polymerase chain reaction-based restrictive fragment length polymorphism (PCR.RFLP) method. Odds ratio (OR) and 95%confidenceinterval (CI) were computed using unconditional logistic regression model. Results GPXl and TXNRD2 polymorphisms individually were not associated with the risk of gastric cancer. Gene-gene interaction of GPXI and TXNRD2 polymorphisms decreased the risk of gastric cancen. Carrying the protective genotype might decrease the risk at 62%(OR=0.38,95%C7=0.26-55.P<0.001) as compared with the risk genotype. Conclusion The GPXl 198 Pro/Pro and TXNRD2 370Arg/Arg genotypes might be associated with the genetic susceptibility of gastric cancer. Key words: Stomach neoplasms;  Glutathione peroxidase;  Thioredoxin reductase;  Polymorphism, single nucleotide