Quantification of Testosterone (T ) by 8 Immunoassays and by Liquid Chromatography -Tandem mass Spectrometry (LC-mSmS) in Normal and Hirsute Women. A multicenter Study Cuantificación de testosterona (t ) por 8 inmunoensayos y por cromatografía líquida en tandem con espectrometría de masa (lC ms/ms). En mujeres normales e hirsutas. Estudio multicéntrico

Objective: To compare T results in normal and hirsute women, obtained by different laboratories employ- ing the same or different methods, including an in-home RIA, and the gold standard method LC-MS/MS. In addition, T results were referred to a curve obtained by 6 different pools that had been prepared on the basis of LC-MS/MS results. Design: Prospective study Setting: Hormone Determination Laboratory, Hospital Italiano, La Plata, and private practice of each participant laboratory. Patient(s): Blood samples were obtained from 78 individuals sorted into 3 groups, namely, normal men (n:39), normal women (n:24) and hirsute women (n:15)