An efficient solid-phase strategy for the construction of chemokines.

Synthesis of chemokines via stepwise SPPS approaches has been shown to be a challenge. Herein, a complete study of different coupling methods, solvents and temperature combined with a continuous-flow synthesizer equipped with feedback monitoring was carried out. The results from this study indicate that this family of molecules can be prepared using an Fmoc/But chemical approach and provide a general method to apply for the elongation of other difficult sequences. Copyright © 2000 European Peptide Society and John Wiley & Sons, Ltd.