Therapeutical effects of polysaccharides spirulina platensis on corneal angiogenesis and the expression of inflammatory factor

Objective To observe the effects of polysaccharides spirulina platensis(PSP) on comeal angiogenesis after alkali burns in mice and investigate the mechanism.Methods With different concentration eyedrops of PSP to mou se with alkali burns on cornea,to observe turbidity of cornea,to detect IL-8and MCP-1 with enzyme linked immunosorbent assay(ELISA),CXCR 1 and CCR 2 B were detected by immunohistochemical method.The experimental data was analyzed by SPSS 13.0 statistical software.Results In group 0.5％PSP,turbidity of cornea,the expression of IL-8 and MCP-1 and their receptor (CXCR 1 and CCR 2 B) were obviously less than group saline (P＜0.05).Conclusion PSP can effectively inhibit the corneal angiogensis by decreasing inflammatory reaction. Key words: Polysaccharides spirulina platensis; Corneal angiogenesis; Inflammatory; Factor,chemotactic; Receptor