Alterations of HSP70 gene expression in rainbow trout (Oncorhyncus mykiss) exposed to deltamethrin

2010 
The aim of the study was to assess the effects of deltamethrin, a synthetic pyrethroid pesticide and potential toxic pollutant to aquatic ecosystems, on the heat shock protein HSP70 gene expression in rainbow trout. Deltamethrin was applied at 0.3 and 0.6 mg/L for 28 days under laboratory conditions. After the experiment, HSP70 was amplified for 20-40 PCR cycles for the control and deltamethrin-treated fish groups. While the quantitative mRNA level was low in the 40th PCR cycle in the control, it was higher in the fish exposed to deltamethrin. The differences between the treated groups and the control group were statistically significant (P < 0.01), but the difference between the treatment groups was not.
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