Producing method of artificial skin and artificial skin

According to one embodiment, comprising a mixture of fibroblasts and collagen dermal simulated layer, culturing after applying the keratinocytes on the dermis simulated layers, the dermis simulated layer keratinocytes in the first culture medium the medium It provides the step of culturing, and the artificial skin manufacturing method comprising culturing the cultured dermal layer simulated by the first medium in a second medium with a DMEM medium and F12 medium mixed.