The Induction and Identification of Differentiation of Human Bone Mesenchymal Stem Cells

Objective To investigate the multilineage differentiation potential of human bone mesenchymal stem cells(hBMSCs),and provide seed cells for transplantation experiments in vivo.Methods The whole bone marrow adherent culture method was used to separate and self-made serum-free culture of hBMSCs;and grew well in,immunofluorescence identification was used to detect the expression of surface antigen CD34,CD44 and CD105 in healthy P4 generation hBMSCs;with different inducers of the medium induced culture,oil red O staining for detection of induced differentiation into fat,alizarin red staining to identify the osteoblast differentiation,alcian blue staining for detection of cartilage induction condition.Results hBMSCs surface antigen CD44,CD105 were positive,CD34 was negative;after the induction culture,oil red O staining,alizarin red staining and alcian blue staining showed positive.Conclusion The isolated cultured hBMSCs are able to differentiate into adipocytes,osteoblasts and chondrocytes,hBMSCs has the potential of multilineage differentiation and can be used as ideal seed cells in vivo transplantation experiments.