Method of manufacturing semi-cortical bone
2010
PURPOSE: A manufacturing method of semi-cortical bone is provided to have the osteo inductivity of a decalcified bone maintaining osteo conductivity of a cortical bone by decalcify only the surface of the cortical bone through the short decalcification time with high concentration of hydrochloric acid. CONSTITUTION: A manufacturing method of semi-cortical bone comprises: a step of washing surface by removing the soft bone tissue; a step of dehydration soaking and mixing the bone tissue with dehydration solution; a degreasing step soaking and mixing the bone tissue with organic solvent; a step decalcifying the bone tissue by acid treatment; and a step of neutralizing the bone tissue by soaking into neutralizing solution. The semi-cortical bone is soaked into the acid of which concentration is 0.5-2.0N, for 1-30 minutes. The acid is selected from the group consisting of hydrochloric acid, formic acid, nitric acid, EDTA, acetic acid and lactic acid.
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