Method for judging place of production of traditional Chinese medicinal pulsatilla by using stable isotope ratio

The invention discloses a method for judging the place of production of traditional Chinese medicinal pulsatilla by using a stable isotope ratio. According to the method, the values of N%, C%, sigma 15N and sigma 13C in a pulsatilla sample are measured by an elemental analysis-stable isotope mass spectrometry technology. The method mainly comprises the following steps of constructing a database including N%, C%, sigma 15N and sigma 13C of the pulsatilla sample in the real place of production by measuring the values of N%, C%, sigma 15N and sigma 13C in the pulsatilla samples in different places of production; measuring the values of N%, C%, sigma 15N and sigma 13C in an unknown place of production; performing Fisher judgment analysis by statistical software, and running a program to judge the real place of production of the pulsatilla sample in the unknown place of production. According to the method disclosed by the invention, the method for accurately judging the place of production of the pulsatilla can be constructed by the elemental analysis-stable isotope mass spectrometry technology, so that the technical difficulty in tracing of the place of production of traditional Chinese medicines in China can be solved; the benefit of a customer can be protected, and the traditional Chinese medicine industry can be developed in a healthy and ordered way.