Development and application of a loop-mediated isothermal amplification assay for detection of Mycoplasma ovipneumoniae

Four primers of Loop-mediated isothermal amplification(LAMP) were designed to establish an assay for detecting Mycoplasma Ovipneumoniae(Mo),which was rapid,easy and low cost.The LAMP was developed after optimizing the reaction condition and system.The specificity,sensitivity and clinical application of the assay were also verified.The results showed that molecular copy count detection of Mo could reached to 1.0×102copies/μL at 60℃ for 60 min by LAMP,with good specificity.As well,detecting to 4 known Mo positive goat lungs was whole positive by LAMP.The LAMP detection of Mo could be used in clinical diagnosis.