Expression of periostin and the effect of hydrocortisone on it in human fibroblasts of scar

Objective:To probe into the role of periostin in the formation of scars and investigate its reaction to hydrocortisone.Methods: Primary fibroblasts were cultured in vitro,then RT-PCR and immunocytochemical technique were used to examine the expressions of mRNA and preotein of periostin respectively in 24 samples of keloid fibroblasts(KFb),hypertrophic scar fibroblasts(HFb) and normal skin fibroblasts(SFb).Results: The mRNA levels of periostin in KFb(1.645±0.549) and HFb(1.084±0.396) were both higher than that in SFb(0.274±0.215,P0.05).After hydrocortisone was added,the mRNA levels of periostin in KFb and HFb were both decreased by 32% and 47% respectively(P0.05);The protein of periostin distributed mainly in the endochylema around the nucleus of fibroblasts.The protein levels of periostin in KFb(91.815±0.961) and HFb(70.166±2.250) were both higher than that in SFb(41.011±1.576,P0.01).Conclusion: The expressions of periostin were increased in the fibroblasts of hyperplasic scars,which could be inhibited by hydrocortisone.periostin may affect the function of fibroblasts of hyperplasic scars.periostin may be a cicatrix specific gene.