The complete chloroplast genome of Gentiana macrophylla

Gentiana macrophylla is a perennial medicinal plant in the family Gentianaceae. Natural sources for G. macrophylla plants are being exhausted due to over-collection and need urgent conservation. The complete chloroplast DNA sequence of G. macrophylla (GenBank accession number: KY856959) was determined in our study. The size of chloroplast genome of G. macrophylla is 149,916 bp, including a large single-copy (LSC) region of 82,911 bp and a small single-copy (SSC) region of 17,095 bp separated by a pair of identical inverted repeat regions (IRs) of 24,955 bp each. A total of 130 genes were successfully annotated containing 86 protein-coding genes, 36 tRNA genes and 8 rRNA genes. The GC content is 37.7%. 33 chloroplast simple sequence repeats (cpSSRs) of Gentiana macrophylla were detected, in which 32 of them are mononucleotide repeats. Phylogenomic analysis strongly supported the close relationship of G. macrophylla and other 5 species in Gentiana.