Preparation and compositions for cancer therapy immune cell-containing composition

Mononuclear NK cells, good balance to differentiate into NKT cells and T cells, to provide a method for growing these cells. The present invention is preferentially a IL-2 and / or IL-15, a first step of culturing the mononuclear cells in a medium containing an anti-CD16 monoclonal antibody, after the first step, mononuclear cells to provide a process for the preparation of an immune cell-containing composition comprising a second step of performing culture under conditions which differentiate into cytotoxic T cells, to. Above after the second step, the IL-2 and / or IL-15, it may also include a third step of performing culture in a medium containing an anti-CD16 monoclonal antibody.