Isomeric Transformation Of 4-Aminobiphenyl by U.V. Radiation and its Influence on the Determination by Flow Injection Analysis with Amperometric and Spectrophotometric Detection.

Abstract Two flow-injection methods for the determination of 4-aminobiphenyl by amperometry (glassy carbon electrode) and spectrophotometry are proposed. A sample volume of 200 or 400 μl containing an analyte concentration of 0.1–1.0 μg ml−1 or 0.8–24.0 μg ml−1 for amperometric or spectrophotometric detection, respectively, is injected into a carrier stream containing 0.04 or 0.02 M Britton-Robinson buffer at pH 10.0 or 8.0, respectively. The throughput thus achieved is 200 (amperometry) and 240 samples/h (spectrophotometry) and the relative standard deviation less than 2.5% and 4.2% respectively. Both methods were applied to the determination of 4-aminobiphenyl in commercially available, legally permitted food colouring additive.