AGGLUTINATION IN GONOCOCCUS INFECTION WITH CONCENTRATED SERUM

The serum of patients with gonococcus infection does not contain, as a rule, agglutinins in sufficient quantity to make the agglutination test of any practical value as an aid in diagnosis. I 1 have found that the arachnoid fluid and serum of syphilitic patients give a more sensitive test when concentrated. The euglobulin and lower pseudoglobulins, obtained by adding saturated solution of ammonium sulphate to the serum, were redissolved in physiologic sodium chlorid solution in a fractional quantity of the original serum. This method of concentration was applied to two antigonococcus rabbit serums made by injecting the Torrey and Buckell 2 gonococcus Strain 34, which has been found to be one of the most widely generalized strains of gonococcus as classified by agglutination and absorption, since it gave clear-cut absorption of agglutinin from twenty-four of twenty-seven monovalent antigonococcus serums. The results of concentration of these two antigonococcus serums are given in