Total plate count and identification of vibrio in pacific white shrimp (Litophenaeus vannamei) from ponds and in those exposed to immunogenic protein membrane Zoothamnium penaei

In shrimp culture, disease is easily caused by the vibrio bacteria. One of the solutions to overcoming this problem is by using an immunogenic protein membrane from Zoothamnium penaei. The purpose of this study was to identify the bacteria Vibrio sp. and to determine the Total Plate Count (TPC) of the bacteria before and after being exposed to the immunogenic protein membrane Zootamnium penaei. This research is expected to resolve the problem of shrimp culture caused by disease. The method of this research was an experimental method; there was a group of shrimp that went without treatment (control) and two treatment groups of shrimp were exposed to an immunogenic protein membrane Zoothamnium penaei concentration of 3ppm and 5ppm. The vanamei shrimp samples were taken from their original habitat of ponds in Lamongan, East Java. The parameters in this study were the identification of conventional bacteria, the Total Plate Count (TPC) and the Survival Rate (SR). The research was held at the Wet Laboratory in the Faculty of Fisheries and Marine, Airlangga University. The results without treatment (control) showed that the bacterium found was Vibrio sp. with a TPC of 3 x 103 CFU/gr. After being exposed to the immunogenic protein membrane for 7 days, there was seen to be the presence of bacterial vibrio sp. The TPC count was lower than before 1 × 105 CFU/gr for the 3 ppm doses and 1 x 104 CFU/gr for the 5ppm doses. The Survival Rate after seven days of treatment was 80%.