Microchip, process of manufacturing the same, and analytical method using the same

The present invention provides a microchip, with a substrate on the substrate and the surrounding housing may contain at least one region of the plurality of liquid samples, reagents and solvents in the surface of the substrate may be a liquid receiving area layer is composed of a monomolecular film covered with a monomolecular film of said liquid accommodating rendered non-affinity region compared to the liquid, the monomolecular film is fixed by a covalent bond with the substrate. The microchip according to the present invention can improve analytical sensitivity, precision of analysis, and detection means can be made compact.