The Chemical Constituents of Tenggulun (Protium Javanicum, Burm, F) Leaf

Protium Javanicum, Burm, F, locally known as Tenggulun, have been used in Balinese traditional culinary and medicines. Although this plant has not received much attention for medicinal uses in Indonesia, the literature on the genera of Protium shows it has worldwide importance source of natural medicines. As a phytochemical study on Indonesian plants, it would be of some interest to investigate the chemical constituents of this plant. The Leaves of Tenggulun collected at Bukit Jimbaran Bali, were air dried, blended to yield a powder and then extracted with ethanol. The crude ethanol extract was then shaken with ether to give two fractions, the ether-soluble fraction A, and the ether insoluble fraction B. The fraction A was first subjected to GC-MS to analyse any volatile compound present and subsequently separated on silica gel column chromatography with gradient elution by increasing polarity using n-hexane, dichloromethane, ethyl acetate, and methanol respectively. While the fraction B was hydrolysed with 1 M HCl. The major components of volatile compounds obtained from Tenggulun leaf were sesquiterpenes i.e: caryophyllene, caryophyllene oxide, and spathulenol. The non-volatile compounds that have been isolated were a series of long chain aliphatic alcohol, a binary mixture of α and s-amyrin, and s-sitosterol. In addition, the compound obtained from hydrolysis of insoluble material was tentatively deduced as a long chain aliphatic diol.