Sex Steroids in the Umbilical Circulation of Fetal Rhesus Monkeys from the Time of Gonadal Differentiation

Male rhesus monkey fetuses have significantly more testosterone (T) in their circulation than females on days 35–50 of gestation (P < 0.01; n = 6 males and 6 females). However, we found no sex differences for androstenedione (Δ4). T concentrations remained significantly higher in male fetuses than in females later in gestation, e.g. days 79–84, 100–133, and 140–160. Levels of Δ4 differed between the sexes only on days 79–84, and dihydrotestosterone concentrations were significantly higher in male fetuses than in females on days 100–133 and 140–163. The fact that Δ4 concentrations were not different betweenthe sexes at the earliest period studied (days 35–50) indicatesthat systemic concentrations of this hormone in the fetus probably are not important for sexual differentiation, especially of the central nervous system. Quantification of three steroids (T,Δ, and dihydrotestosterone) in umbilical arterial and venous plasma from five male and nine female fetuses (days 35–100) revealed significant arterial/ve...