Prokaryotic Expression of Chicken Infectious Anemia Virus VP1

According to the sequences of CAV VP1 published GenBank,a series of primers were designed. Four different segments of VP1 genes were amplified through PCR,and had been inserted into the multiple cloning site of expression plasmid vector pET28a.Four kinds of recombinant expression engineering bacterium(E.coli BL21/pET-28-CAVVP1,E.coli BL21/pET-28-CAVVPlNd29, E.coli BL21/pET-28-CAVVP1Nd56,E.coli BL21/pET-28-CAV VPlNd137) had been constructed.The expression of full VP1 and VP1 with a serial of N-terminus truncated of chicken infectious anemia virus(CAV) in recombinant E.coli were studied,the best way of expression were selected throgh SDS-PAGE electrophoresis.The results showed that E.coli BL21/ pET-28-CAV VPlNd29 can express a soluble protein of 44 ku,E.coli BL21/pET-28-CAV VPlNd56 can express an insoluble protein of 40 ku,but no visible expression protein can be seen in the product of induced recombinant E.coli BL21/pET-28-CAV VP1 and E.coli BL21/pET-28-CAV VP1Nd137.The soluble expression protein of CAV VP1Nd29 can be used in development of antigens, which will be used in the produce of diagnosis agent and subunit vaccine of CAV.