DNA damage repair Neil3 gene Knockout in MOLT-4

RNAi is superannuated cellular mechanism that protect organism against viruses that replicate through double- stranded RNA. RNAi can be used to diminish gene expression from plasmid expressing and inserted sequence repeat. A stable harpin would be expressed after the vector was integrated into the genome. In this paper a shiRNA expressing vector for Neil3 was designed and developed which is capable of replication in MOLT-4. This shiRNA vector had the ability to arose the RNAi pathway, and reduce the gene expression of Neil3. This was assessed by using pSilence 4.1CMV as a vector, and Gapdh as positive control.