A Quartz Crystal Microbalance Study of Polycation-Supported Single and Double Stranded DNA Surfaces

A quartz crystal microbalance with dissipation monitoring (QCM-D) was used to investigate the properties and formation of a genomic mammalian DNA surface on a polycationic poly(ethylenimine) (PEI) film. We show that both single- and double-stranded DNA films can be deposited on the PEI surface by modulating the DNA adsorption time. The two distinct DNA surfaces can be confirmed by their interactions with urea, a common DNA denaturant, and ethidium bromide, a common DNA intercalator, both of which lead to characteristic changes in the QCM-D frequency and dissipation. The hybridization process between surface-bound single-stranded DNA to complementary strands in solution can be resolved in real-time. Moreover, we have also investigated the effects of incorporating NaCl in the various PEI-DNA assemblies and have shown that higher ionic strengths lead to greater DNA adsorption to the PEI surface. An increase in the QCM-D resonant frequency and a decrease in dissipation occur when these assemblies are rinsed w...