Chlorella Vulgaris Modulates Hydrogen Peroxide-Induced Dna Damage AndTelomere Shortening Of Human Fibroblasts Derived From Different AgedIndividuals
2009
The objective of this study was to investigate the modulatory effect of
Chlorella Vulgaris on cultured fibroblast cells derived from young
and old aged individuals focusing on DNA damage, telomere length and
telomerase activity. Dose-response test of the algal extract on cells
in both age groups revealed that optimum viability was observed at a
concentration of 50 μg/ml. Results obtained showed that Chlorella
vulgaris exhibited protective effects against H2O2-induced oxidative
stress as shown by the reduction in damaged DNA caused by H2O2
treatment (p<0.05) in Chlorella vulgaris pre- and post-treated
groups (p<0.05). Pre-treatment of Chlorella vulgaris resulted in a
significant decrease in DNA damage suggesting a bioprotective effect
against free radical attacks. A decline in DNA damage was observed in
post-treated cells which proves Chlorella vulgaris to present
bioremediative properties. In cells induced with oxidative stress,
telomere length decreased significantly coupled with a concomitant
decline of telomerase activity (p<0.05). However, these reductions
were prevented with prior and post treatment of Chlorella vulgaris.
Therefore, we concluded that Chlorella vulgaris exhibited bioprotective
effects especially in cells obtained from young donor but were more
bioremediative for cells obtained from old donor as indicated by DNA
damage, telomere shortening and reduction in telomerase activity.
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