Tomographie diffractive sans lentille appliquée à la culture cellulaire 3D

The study of in vitro cell populations is a major concern for biologists in the realms of drug screening and oncology for example. With standard microscopy techniques, crucial information at the mesoscopic scale is still difficult to reach due to reduced field of view and complexity to monitor cell behaviors in real time over large periods of time. Lensfree video microscopy has already overcome these difficulties in the context of 2D cell biology. Based on the in-line holography principle, it puts cells directly between a coherent light source and a sensor allowing large field of view, e.g. 30 mm 2. With the growth of 3D cell cultures, the new challenging task is to extend this imaging technique to acquisitions and 3D reconstructions of cellular culture. For this purpose, we have developed an experimental optical bench dedicated to lensfree diffractive tomography and studied several 3D reconstruction algorithms on acquisitions of 3D prostatic cell cultures.