CRISPR (clustered regularly interspaced short palindromic repeats)-Cas9-system-based Saccharomyces cerevisiae genome concurrent multiplex edition vector and application thereof

The invention relates to a CRISPR (clustered regularly interspaced short palindromic repeats)-Cas9-system-based Saccharomyces cerevisiae genome concurrent multiplex edition vector and application thereof. Cas9 protein expression cassettes and a plurality of sgRNA (small guide ribonucleic acid) scaffold expression cassettes are integrated into the vector to obtain a vector-Cas9-sgRNA scaffold, and a plurality of sgRNA segments designed and synthesized by using ADE1 as a target are integrated into the vector-Cas9-sgRNA scaffold, thereby obtaining the Saccharomyces cerevisiae genome concurrent multiplex edition vector. By adopting a simple substance granule single-copy vector system, one plasmid is utilized to simultaneously express the Cas9 protein and three sgRNA scaffolds, so that three target genes can be edited simultaneously; and thus, the multigene edition operation is very simple. Besides, the target gene edition system only needs one-step component and conversion, and thus, is simple to operate.