Nucleotide sequence analysis for a new HLA-B allele HLA-B＊13 ： 01： 06

Objective To confirm a new allele HLA B＊13： 01：06 and analyze its nucleotide sequence. Methods Genomie DNA was extracted using a Qiagen DNA extraction kit. Nueleotide sequences of HLA A, HLA-B, HLA-C and HLA-DRB1 were analyzed by polymerase chain reaction-sequence based typing （PCR-SBT）. HLA high-resolution results were assigned, and the nucleotide sequences of HLA B locus was compared with that of HLA-3＊13 ： 01 ： 01. Results The nucieotide sequence of the new allele shows a strong similarity to that of HLA-B＊13 = 01：01. One nucleotide in exon 2 has changed from G→A at position 219 （codon 49 GCG→GCA）, which however did not result in amino acid change. Conclusion The novel allele verified by sequencing has been submitted to GenBank and officially named as HLA-B＊13： 01： 06 by the World Health Organization HLA Nomenclature Committee. Key words: Allele;  HLA B ;  13：01：06;  Sequencing-based typing