COMPARATIVE STUDY ON DEGRADATION OF HEMOGLOBINS IN MOUSE AND MICROTUS FORTIS BY SCHISTOSOMA JAPONICUM DIGESTING PROTEINASES

Aim in order to do research work on Histiding rich protein2 (HRP2) of Plasmodium falciparum yunan line for malaria diagnosis and Vaccine, HRP2 gene was prokaryoticly expressed.Methods .HRP2 gene rframent was cloned into prokaryotic expression vector pGEX-4T-1 to form pGEX-4T-1/HRP2, E. coli BL21 was transformed.After induerement the expresion product was purified and used to inununize BALB/C mice. Immunochromatographic test and Westem blot were done.Result pGEX-4T-1/HRP2 successfully constructed.After inducement,a GST fusion protein (about 60KD) was expressed,which can be recognized by HRP2 specific Meanwhile, the parasite HRP2 antien insied the infected red blood cells (IRBC) was detected by mice antisera in Westem blot.Conclusion HRP2 gene was successfully expressed,a good basis for future research was built up.