THE EFFECTS OF LOW CALORIC COMMERICAL SWEETENER "MARDIN " AND SUGAR ON SERUM GLUCOSE AND LIPID PROFILELS IN RATS

Sucralose is a non-nutritional artificial sweetener with a chemical formula C12H19CL3O8. Sucralose is the only non-caloric sweetener, which is derived from sugar or sucrose. Sucralose is sweeter than sugar 600 times and has high stability against heat. Mardin as a commercial sweetener in addition to sucralose has various compounds consisting lactose, L-Leucine, Cross, carmolase sodium and PVP also produces low rate calorie. Therefore, this research was conducted on the impact of Mardin on serum glucose and lipid profiles in rats. In this study, 18 male Wistar rats weighing about 220 ± 20g were divided into 3 groups each of which consisted of 6 rats. The groups were as follows: control group, sucralose and sugar group. Controls received no intervention. Sucralose group received Mardin (sucralose) with a Dose of 15 mg / kg daily for one month by gavage. Due to the amount of commercial sweetener tablets, which is equal to 2.7 grams of sugar, it was calculated for daily use of sugar group for one month by gavage. At the end of the period, blood sampling and serum separation was done; then, serum glucose and lipid profiles, including HDL, LDL, and total cholesterol and triglyceride measurements were conducted using diagnostic kits and spectrophotometric methods. The results were analyzed by SPSS (version 18.0) and a nonparametric Kruskal-Wallis test. The results revealed that there was no meaningful difference about understudied factors, so it can be concluded that Mardin has no adverse effect on understudied biochemical markers hence it is not prohibited for obese and diabetics.