Cannula-based computational fluorescence microscopy

We converted a solid-glass cannula into a high-resolution widefield fluorescence microscope. Calibrating the space-variant point-spread functions of the cannula and applying a nonlinear optimization algorithm to reconstruct object details enable this development. The resolution of our system is ∼1 μm, and fluorophore position is determined to a precision of ∼20 nm. Images of microglia from fixed slices of mouse brains at various post-natal development stages were also obtained.