Isolation of Glycoalkaloids with the Chromatotron and Their Determination by High-Performance Liquid Chromatography

Abstract An extract of Solanum laciniatum leaves was fractionated with the Chromatotron. The glycoalkaloid fraction thus obtained was analyzed by reversed-phase HPLC. The load capacity of the Chromatotron with a 2-mm layer thickness is 600 mg (as determined with cholesterol) and the recovery of solasonine from the plant extract (as determined by HPLC) is 93–96%. The HPLC method permits the detection of as little as 1.5 μg solasonine or 3.5 μg solamargine with a linear detector response up to 375 μg for the former and 250 μg for the latter.