Analytical Methodologies for the Determination of Midazolam in Pharmaceuticals

Benzodiazepines are drugs with hypnotic, anticonvulsant and tranquilizing properties and are prescribed worldwide for the therapy of anxiety, sleep disorders, and convulsive attacks. Midazolam (MDZ) is a commonly used benzodiazepine in clinical practice. Numerous methods have been reported for their determination in different types of samples. The aim of this paper is to review the different methods validated for the establishment of the stability of pharmaceutical preparations so they can be used to study samples used in clinical practice that are not covered in the official label information of the product. This review covers a selection of the literature published on determination of midazolam in pharmaceutical dosage forms from the 1990s decade. The main goal of most research on analytical determinations of MDZ in pharmaceutical preparations is to establish the stability of the drug. The aim of these studies was to set up and validate an analytical method, able to separate and quantify the drug mixtures under study to assess both the compatibility and the stability of MDZ when mixed with other drugs, and to provide recommendations for the appropriate storage conditions.